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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
08/01/2024 |
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Data da última atualização: |
08/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PRADO, G. S.; ROCHA, D. C.; SANTOS, L. N. dos; CONTILIANI, D. F.; NOBILE, P. M.; MARTINATI-SCHENK, J. C.; PADILHA, L.; MALUF, M. P.; LUBINI, G.; PEREIRA, T. C.; MONTEIRO-VITORELLO, C. B.; CRESTE, S.; BOSCARIOL-CAMARGO, R. L.; TAKITA, M. A.; CRISTOFANI-YALY, M.; SOUZA, A. A. de. |
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Afiliação: |
GUILHERME SOUZA PRADO, INSTITUTO AGRONÔMICO; DHIÔVANNA CORRÊIA ROCHA, UNIVERSIDADE ESTADUAL DE CAMPINAS; LUCAS NASCIMENTO DOS SANTOS, INSTITUTO AGRONÔMICO; DANYEL FERNANDES CONTILIANI, INSTITUTO AGRONÔMICO; PAULA MACEDO NOBILE, INSTITUTO AGRONÔMICO; JULIANA CAMARGO MARTINATI-SCHENK, INSTITUTO AGRONÔMICO; LILIAN PADILHA, CNPCa; MIRIAN PEREZ MALUF, CNPCa; GREICE LUBINI, INSTITUTO AGRONÔMICO; TIAGO CAMPOS PEREIRA, UNIVERSIDADE DE SÃO PAULO; CLAUDIA BARROS MONTEIRO-VITORELLO, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; SILVANA CRESTE, INSTITUTO AGRONÔMICO; RAQUEL LUCIANA BOSCARIOL-CAMARGO, INSTITUTO AGRONÔMICO; MARCO AURÉLIO TAKITA, INSTITUTO AGRONÔMICO; MARIÂNGELA CRISTOFANI-YALY, INSTITUTO AGRONÔMICO; ALESSANDRA ALVES DE SOUZA, INSTITUTO AGRONÔMICO. |
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Título: |
CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 14, article 1331258, 2023. |
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DOI: |
https://doi.org/10.3389/fpls.2023.1331258 |
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Idioma: |
Inglês |
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Conteúdo: |
Gene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it has allowed the expansion of possibilities in plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA editing, prime editing, and nucleic acid probing or detection. This technology mostly depends on in vitro tissue culture and genetic transformation/transfection protocols, which sometimes become the major challenges for its application in different crops. Agrobacterium-mediated transformation, biolistics, plasmid or RNP (ribonucleoprotein) transfection of protoplasts are some of the commonly used CRISPR delivery methods, but they depend on the genotype and target gene for efficient editing. The choice of the CRISPR system (Cas9, Cas12), CRISPR mechanism (plasmid or RNP) and transfection technique (Agrobacterium spp., PEG solution, lipofection) directly impacts the transformation efficiency and/or editing rate. Besides, CRISPR/Cas technology has made countries rethink regulatory frameworks concerning genetically modified organisms and flexibilize regulatory obstacles for edited plants. Here we present an overview of the state-of-the-art of CRISPR technology applied to three important crops worldwide (citrus, coffee and sugarcane), considering the biological, methodological, and regulatory aspects of its application. In addition, we provide perspectives on recently developed CRISPR tools and promising applications for each of these crops, thus highlighting the usefulness of gene editing to develop novel cultivars. MenosGene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it has allowed the expansion of possibilities in plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA editing, prime editing, and nucleic acid probing or detection. This technology mostly depends on in vitro tissue culture and genetic transformation/transfection protocols, which sometimes become the major challenges for its application in different crops. Agrobacterium-mediated transformation, biolistics, plasmid or RNP (ribonucleoprotein) transfection of protoplasts are some of the commonly used CRISPR delivery methods, but they depend on the genotype and target gene for efficient editing. The choice of the CRISPR system (Cas9, Cas12), CRISPR mechanism (plasmid or RNP) and transfection technique (Agrobacterium spp., PEG solution, lipofection) directly impacts the transformation efficiency and/or editing rate. Besides, CRISPR/Cas technology has made countries rethink regulatory frameworks concerning genetically modified organisms and flexibilize regulatory obstacles for edited plants. Here we present an overview of the state-of-the-art of CRISPR technology applied to three important crops worldwide (citrus, coffee and sugarcane), considering the biological, methodological, and regulatory aspects of its applica... Mostrar Tudo |
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Thesaurus Nal: |
Citrus; Coffee beans; Genome; Perennials; Sugarcane. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160555/1/CRISPR-technology-towards.pdf
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Marc: |
LEADER 02801naa a2200373 a 4500
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024 7 $ahttps://doi.org/10.3389/fpls.2023.1331258$2DOI
100 1 $aPRADO, G. S.
245 $aCRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane.$h[electronic resource]
260 $c2023
520 $aGene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it has allowed the expansion of possibilities in plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA editing, prime editing, and nucleic acid probing or detection. This technology mostly depends on in vitro tissue culture and genetic transformation/transfection protocols, which sometimes become the major challenges for its application in different crops. Agrobacterium-mediated transformation, biolistics, plasmid or RNP (ribonucleoprotein) transfection of protoplasts are some of the commonly used CRISPR delivery methods, but they depend on the genotype and target gene for efficient editing. The choice of the CRISPR system (Cas9, Cas12), CRISPR mechanism (plasmid or RNP) and transfection technique (Agrobacterium spp., PEG solution, lipofection) directly impacts the transformation efficiency and/or editing rate. Besides, CRISPR/Cas technology has made countries rethink regulatory frameworks concerning genetically modified organisms and flexibilize regulatory obstacles for edited plants. Here we present an overview of the state-of-the-art of CRISPR technology applied to three important crops worldwide (citrus, coffee and sugarcane), considering the biological, methodological, and regulatory aspects of its application. In addition, we provide perspectives on recently developed CRISPR tools and promising applications for each of these crops, thus highlighting the usefulness of gene editing to develop novel cultivars.
650 $aCitrus
650 $aCoffee beans
650 $aGenome
650 $aPerennials
650 $aSugarcane
700 1 $aROCHA, D. C.
700 1 $aSANTOS, L. N. dos
700 1 $aCONTILIANI, D. F.
700 1 $aNOBILE, P. M.
700 1 $aMARTINATI-SCHENK, J. C.
700 1 $aPADILHA, L.
700 1 $aMALUF, M. P.
700 1 $aLUBINI, G.
700 1 $aPEREIRA, T. C.
700 1 $aMONTEIRO-VITORELLO, C. B.
700 1 $aCRESTE, S.
700 1 $aBOSCARIOL-CAMARGO, R. L.
700 1 $aTAKITA, M. A.
700 1 $aCRISTOFANI-YALY, M.
700 1 $aSOUZA, A. A. de
773 $tFrontiers in Plant Science$gv. 14, article 1331258, 2023.
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Registro original: |
Embrapa Café (CNPCa) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
06/03/2008 |
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Data da última atualização: |
06/03/2008 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
AMARAL, A. M. do; HOMEM, R. A.; MACHADO, M. A. |
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Afiliação: |
Alexandre Morais do Amaral, Embrapa Recursos Genéticos e Biotecnologia; R. A. Homem, Centro APTA Citros "Sylvio Moreira" - IAC; M. A. Machado, Centro APTA Citros "Sylvio Moreira" - IAC. |
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Título: |
Expressão de genes de Xanthomonas axonopodis pv. citri através de interação com a planta de citros. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Fitopatologia Brasileira, Brasília, DF, v. 32, supl., p. S247, ago. 2007. Resumo 0692. |
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Idioma: |
Português |
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Notas: |
Edição dos Resumos do XL Congresso Brasileiro de Fitopatologia, Maringá, PR, ago. 2007. |
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Thesagro: |
Cancro Cítrico; Doença de Planta. |
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Thesaurus NAL: |
Xanthomonas axonopodis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00672naa a2200181 a 4500
001 1173591
005 2008-03-06
008 2007 bl uuuu u00u1 u #d
100 1 $aAMARAL, A. M. do
245 $aExpressão de genes de Xanthomonas axonopodis pv. citri através de interação com a planta de citros.
260 $c2007
500 $aEdição dos Resumos do XL Congresso Brasileiro de Fitopatologia, Maringá, PR, ago. 2007.
650 $aXanthomonas axonopodis
650 $aCancro Cítrico
650 $aDoença de Planta
700 1 $aHOMEM, R. A.
700 1 $aMACHADO, M. A.
773 $tFitopatologia Brasileira, Brasília, DF$gv. 32, supl., p. S247, ago. 2007. Resumo 0692.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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